HOCL Studies

International Journal of Molecular Sciences 17.7 (2016): 1161

Effectiveness of hypochlorous acid to reduce the biofilms on titanium alloy surfaces in vitro

Journal of Endodontics 42.7 (2016): 1120-1125

Bactericidal Effect of Strong Acid Electrolyzed Water against Flow Enterococcus faecalis Biofilms

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 107.2 (2009): 295-298

Antimicrobial efficacy of 4.2% sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in infected human root canals

Microbe(s): Escherichia coli, Porphyromonas gingivalis, Enterococcus faecalis, Streptococcus sanguinis

ABSTRACT - Full Text PDF 223 PDF
Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability ana...

Microbe(s): Enterococcus faecalis

ABSTRACT - Full Text PDF 254
Introduction This study evaluated the bactericidal effect of strong acid electrolyzed water (SAEW) against flow Enterococcus faecalis biofilm and its potential application as a root canal irrigant. Methods Flow E. faecalis biofilms were generated under a constant shear flow in a microfluidic system. For comparison, static E. faecalis biofilms were generated under a static condition on coverslip surfaces. Both the flow and static E. faecalis biofilms were treated with SAEW. Sodium hypochlorite (N...

Microbe(s): Enterococcus faecalis

ABSTRACT - Full Text PDF 35
Objective: The purpose of this study was to determine the antimicrobial efficacy of sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in human root canals infected by Enterococcus faecalis. Study design: One hundred sixty-five human single-rooted teeth were prepared and inoculated with E. faecalis for 48 h. Teeth were divided into 3 experimental groups according to the irrigation pattern used: group 1, 4.2% NaOCl pH 12; group 2, 4.2% NaOCl pH 7.5; and group 3, 4.2% NaOCl pH 6.5. Samples from t...

JOURNAL OF MICROBIOLOGY-SEOUL- 44.4 (2006): 417

Antibacterial effect of electrolyzed water on oral bacteria

Dental materials journal 21.2 (2002): 93-104

Durability of bactericidal activity in electrolyzed neutral water by storage

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 87.1 (1999): 83-87

Bactericidal effect of electrolyzed neutral water on bacteria isolated from infected root canals

Microbe(s): Total Microbial Count

ABSTRACT - Full Text PDF 76
This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Puri-w...

Microbe(s): Staphlycoccus Aureus

ABSTRACT - Full Text PDF 78
Electrolyzed strong and weak acid waters have been widely used for sterilization in clinical dentistry because of their excellent bactericidal activities. Electrolyzed neutral water was recently developed with a new concept of long-term good durability in addition to the excellent bactericidal activity similar to acid waters. The present study, evaluated the storage life of this water compared with the acid waters in terms of the changes in pH, oxidation-reduction potential (ORP), residual chlor...

Microbe(s): Total Microbial Count

ABSTRACT - Full Text PDF 67
Objective. The purposes of this study were to examine the time-related changes in pH, oxidation-reduction potential, and concentration of chlorine of electrolyzed neutral water and to evaluate the bactericidal effect of electrolyzed neutral water against bacteria from infected root canals. Study Design. Various properties of electrolyzed neutral water pH value, oxidation-reduction potential, and concentration of chlorine were measured at different times after storage of the water in the open sta...

Infection Control & Hospital Epidemiology 35.12 (2014): 1505-1510

Evaluating use of neutral electrolyzed water for cleaning near-patient surfaces

Canadian Journal of Microbiology 58.4 (2012): 448-454

Efficacy of acidic and basic electrolyzed water in eradicating Staphylococcus aureus biofilm

Applied and environmental microbiology 73.14 (2007): 4463-4468

Evaluation of liquid-and fog-based application of Sterilox hypochlorous acid solution for surface inactivation of human norovirus

Microbe(s): Total Microbial Count, methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA)

ABSTRACT - Full Text PDF 184
This study aimed to monitor the microbiological effect of cleaning near-patient sites over a 48-hour period with a novel disinfectant, electrolyzed water. One ward dedicated to acute care of the elderly population in a district general hospital in Scotland. Lockers, left and right cotsides, and overbed tables in 30 bed spaces were screened for aerobic colony count (ACC), methicillin-susceptible Staphylococcus aureus (MSSA), and methicillin-resistant S. aureus (MRSA) before cleaning with electr...

Microbe(s): Staphlycoccus Aureus

ABSTRACT - Full Text PDF 170
Staphylococcus aureus is a major pathogen. It can form biofilm on the surfaces of medical devices and food equipment, which makes it more difficult to eradicate. To develop a novel method to eradicate S. aureus biofilm, the effects of electrolyzed water on removing and killing S. aureus biofilm were investigated in this study. By using a biofilm biomass assay with safranin staining and visualization of biofilm architecture with scanning electron microscopy, it was shown that basic electrolyzed w...

Microbe(s): Viruses, Norovirus

ABSTRACT - Full Text PDF 88
Noroviruses (NVs) are the most frequent cause of outbreaks of gastroenteritis in common settings, with surface-mediated transfer via contact with fecally contaminated surfaces implicated in exposure. NVs are environmentally stable and persistent and have a low infectious dose. Several disinfectants have been evaluated for efficacy to control viruses on surfaces, but the toxicity and potential damage to treated materials limits their applicability. Sterilox hypochlorous acid (HOCl) solution (HAS)...

Artificial Organs 28.6 (2004): 590-592

The Bactericidal Effects of Electrolyzed Oxidizing Water on Bacterial Strains Involved in Hospital Infections

Bulletin of Osaka Medical College 48 (2003): 29-36

A novel electrolyzed sodium chloride solution for the disinfection for dried HIV-1

International Journal of Molecular Sciences 17.7 (2016): 1161

Effectiveness of hypochlorous acid to reduce the biofilms on titanium alloy surfaces in vitro

Microbe(s): Multiple

ABSTRACT - Full Text PDF 80
The study is designed to investigate bactericidal actions of electrolyzed oxidizing water on hospital infec-tions. Ten of the most common opportunistic pathogens are used for this study. Cultures are inoculated in 4.5 mL of electrolyzed oxidizing (EO) water or 4.5 mL of sterile deionized water (control), and incubated for 0, 0.5, and 5 min at room temperature. At the exposure time of 30 s the EO water completely inactivates all of the bacterial strains, with the exception of vegetative cells and...

Microbe(s): Viruses, HIV

ABSTRACT - Full Text PDF 106
Electrolyzed products of a sodium chloride solution contain free residual chlorine and have been proved to be effective for disinfection. Electrolyzed strong acid water containing a low sodium chloride concentration (ESW-L) is prepared by the electrolysis of a solution containing a low sodium chloride concentration (0.1% or less). Although ESW-L has been confirmed to be an effective disinfectant, disinfective efficacy against dried HIV-1 and a target of ESW-L against HIV-1 have not been clarifie...

Microbe(s): Escherichia coli, Porphyromonas gingivalis, Enterococcus faecalis, Streptococcus sanguinis

ABSTRACT - Full Text PDF 223
Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability ana...

Wounds 27.10 (2015): 265-273

A new acid-oxidizing solution: assessment of its role on methicillin-resistant Staphylococcus aureus (MRSA) biofilm morphological changes

PloS one 8.2 (2013): e55118

Direct electric current treatment under physiologic saline conditions kills Staphylococcus epidermidis biofilms via electrolytic generation of hypochlorous acid

Canadian Journal of Microbiology 58.4 (2012): 448-454

Efficacy of acidic and basic electrolyzed water in eradicating Staphylococcus aureus biofilm

Microbe(s): Methicillin-resistant Staphylococcus aureus, MRSA

ABSTRACT - Full Text PDF 225
OBJECTIVE: Biofilms represent a key challenge in the treatment of chronic wounds, as they are among the main reasons for delays in chronic wound healing. This in vitro study was aimed at evaluating the activity of a new acid-oxidizing solution (AOS) on biofilm formation. Acid-oxidizing solution contains free chlorine species with stabilized hypochlorous acid in high concentration (> 95) and is RP2). Different approaches were used to assess the prevention and eradication of methicillin-resist...

Microbe(s): Staphylococcus epidermidis

ABSTRACT - Full Text PDF 16
The purpose of this study was to investigate the mechanism by which a direct electrical current reduced the viability of Staphylococcus epidermidis biofilms in conjunction with ciprofloxacin at physiologic saline conditions meant to approximate those in an infected artificial joint. Biofilms grown in CDC biofilm reactors were exposed to current for 24 hours in 1/10th strength tryptic soy broth containing 9 g/L total NaCl. Dose-dependent log reductions up to 6.7 log10 CFU/cm2 were observed with t...

Microbe(s): Staphylococcus aureus

ABSTRACT - Full Text PDF 170
Staphylococcus aureus is a major pathogen. It can form biofilm on the surfaces of medical devices and food equipment, which makes it more difficult to eradicate. To develop a novel method to eradicate S. aureus biofilm, the effects of electrolyzed water on removing and killing S. aureus biofilm were investigated in this study. By using a biofilm biomass assay with safranin staining and visualization of biofilm architecture with scanning electron microscopy, it was shown that basic electrolyzed w...

Hypochlorous Acid: A Review